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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Dev Cell. 2008 Oct;15(4):603–616. doi: 10.1016/j.devcel.2008.08.005

Figure 5. cdc-48.3(RNAi) leads to mitotic delays and spindle defects.

Figure 5

A) Embryos from OD57 (mCherry∷Histone H2B; GFP∷α-tubulin) L4 hermaphrodites fed control and cdc-48.3(RNAi) were subjected to live imaging. Time 0 (panels c and b’) corresponds to metaphase chromosome alignment. a, a’) pronuclear meeting (-390 and -450 seconds respectively from metaphase), b) prophase, b’) metaphase chromosome alignment, c) metaphase chromosome alignment and completion of spindle rotation, c’) completion of spindle rotation, d, d’) anaphase, e, e’) telophase, f, f’) metaphase of AB cell division. Scale bar=10 μm. B) Graph of mitotic progression in control (n=4) and cdc-48.3(RNAi) (n=4) treated OD57 embryos undergoing the first mitotic division. AB Metaphase: metaphase of the AB cell (two-cell embryo). C) Wt and air-2(or207ts) L4 hermaphrodites were subjected to mock or cdc-48.3 dsRNA microinjection, and reared at 15°C or 22°C. Embryonic viability was scored 48 hours post-injection. Wt control 15°C, n=992, 22°C, n=1002; wt cdc-48.3(RNAi) 15°C, n=1123, 22°C, n=1099; air-2(or207ts) control 15°C, n=1055, 22°C, n=1048; air-2(or207ts);cdc-48.3(RNAi) 15°C, n=1068, 22°C, n=1063. D) Live imaging of embryos from OD57 hermaphrodites subjected to mock or cdc-48.3 dsRNA microinjection was performed (control, n=3 embryos; cdc-48.3(RNAi), n=7 embryos). Time 0 (c and c’): metaphase chromosome alignment. a, a’) pronuclear meeting (-330 and -630 seconds respectively from metaphase), b) prophase, b’) metaphase chromosome alignment, c) metaphase chromosome alignment and completion of spindle rotation, c’) completion of spindle rotation, d, d’) anaphase, e, e’) telophase, f, f’) metaphase of AB cell division. Scale bar=10 μm. E) Graph of mitotic progression in one-cell embryos from control (n=3) and cdc-48.3 dsRNA (n=5) injected OD57 hermaphrodites. AB Metaphase: metaphase of the AB cell (two-cell embryo).