Table 2.
Microinjection of cdc-48.3 dsRNA leads to mitotic defects
| Stage* | >2 Centrosomes | Unattached chromosomes | Chromosome congression defects | Chromosome segregation defects |
|---|---|---|---|---|
| Prophase | ||||
|
control(RNAi)
(n=11) |
0.00% | 0.00% | 0.00% | N/A |
|
cdc-48.3(RNAi)
(n=13) |
15.38% | 7.69% | 30.77% | N/A |
| Prometaphase | ||||
|
control(RNAi)
(n=14) |
0.00% | 0.00% | 0.00% | N/A |
|
cdc-48.3(RNAi)
(n=10) |
20.00% | 10.00% | 30.00% | N/A |
| Metaphase | ||||
|
control(RNAi)
(n=7) |
0.00% | 0.00% | 0.00% | N/A |
|
cdc-48.3(RNAi)
(n=7) |
14.29% | 28.57% | 57.14% | N/A |
| Anaphase | ||||
|
control(RNAi)
(n=26) |
0.00% | 0.00% | N/A | 0.00% |
|
cdc-48.3(RNAi)
(n=22) |
22.73% | 9.09% | N/A | 45.45% |
| Telophase | ||||
|
control(RNAi)
(n=16) |
0.00% | 0.00% | N/A | 0.00% |
|
cdc-48.3(RNAi)
(n=17) |
11.76% | 17.65% | N/A | 41.18% |
| Late Telophase/G1 | ||||
|
control(RNAi)
(n=24) |
0.00% | 0.00% | N/A | 0.00% |
|
cdc-48.3(RNAi)
(n=23) |
26.09% | 17.39% | N/A | 56.52% |
*
Embryos from mock and cdc-48.3 dsRNA microinjected L4 hermaphrodites were fixed and stained with DAPI and α-tubulin antibodies. Mitotic defects in one-cell embryos at various mitotic stages were tabulated. Similar defects were also present in older embryos.