Table 1.

List of primers, gene specificity, nucleotide position, sequence, and source

Primer	Gene	Position	Nucleotide position	1st Round	2nd Round	Sequence	Source
Zfy2F	Zfy	5′ Intron	−459–439	X		5′-tgtcagcataagcaggct	2
Zfy1F	Zfy	5′ Intron	−166–149	X		5′-tggagtttgctgttacct	2
Zfx2F	Zfx	5′ Intron	−731–717	X		5′-gcttctgtgactga	2
Zfx1F	Zfx	5′ Intron	−403–386	X		5′-tgggtttaagctctactg	2
Zf2F	Zfy/x	5′ Intron	−804–783	X		5′-ttccaggcagtac	2
δZfyF	Zfy	Exon	292–315	X	X	5′-tgcataaggaaaaagccaaca	1
δZfxF	Zfx	Exon	292–315	X	X	5′-tgcataaggagaaaggagccaaca	1
δZfyR	Zfy	Exon	315–292		X	5′-tgttggctttttctttatgca	1
δZfxR	Zfx	Exon	315–292	X	X	5′-tgttggctcctttctccttatgca	1
Exon4F	Zfy/x	Exon	885–900		X	5′-cgccatattctctcag	1
Exon4R	Zfy/x	Exon	900–885		X	5′-ctgagagaatatggcg	1
olw111F	Zfy/x	Exon	1005–1028		X	5′-agtgtaccagtgtgagtactgtgagtat	3
olw111R	Zfy/x	Exon	1028–1005		X	5′-atactcacagtactcacactgata	3
Exon5F	Zfy/x	Exon	1057–1071		X	5′-ggcacgttatctcca	1
Exon5R	Zfy/x	Exon	1071–1057		X	5′-tggagataacgtgcc	1
Exon6R	Zfy/x	Exon	1181–1164	X	X	5′-gggcaggccaacttcctt	1
olw144	Zfy	3′ Flank	(+)195–165	X		5′-agaaactgaataggattcagaacagcg	3
olw143	Zfx	3′ Flank	(+)195–165	X		5′-aacaaactgaatagaattcagaacacac	3