Table 1. Patient characteristics of training and test studies.

Diagnosis Group	Description	Total 4	Gender		Age					Location 1
			Male	Female	<50	50–59	60–69	≥70	Median5	Distal	Proximal
Training Set
Colorectal Cancer	Stage I	63	30	33	9	16	18	20	61	41	21
	Stage II	83	46	37	10	28	28	17	61	50	33
	Stage III	59	37	22	2	22	26	9	60	47	10
	Stage IV	29	16	13	5	8	10	6	60	21	6
	Stage NA	19	15	4	2	5	8	4	64	14	5
Adenomas/Polyps	<10 mm	53	27	26	10	13	16	14	61	34	19
	≥10 mm	65	37	28	38	25	2	0	47	62	3
Healthy/Normal Colon		102	36	66	19	34	27	22	59
Non-colorectal Cancer2		60	34	25	11	18	20	11	61
Non-cancerous Disease 3		167	63	103	71	39	38	19	52
Total		700	341	357	177	208	193	122	58	269	97
Test Set
Colorectal Cancer	Stage I	22	15	7	0	4	6	10	69	11	1
	Stage II	37	23	14	1	8	13	11	67	11	8
	Stage III	54	27	27	2	10	17	20	67	26	7
	Stage IV	11	8	3	0	4	4	3	63	4	0
	Stage NA	3	3	0	1	1	0	0	49	1	0
Adenomas/Polyps	<10 mm	34	21	13	0	4	19	11	68	0	0
	≥10 mm	17	14	3	1	4	9	3	66	0	0
Healthy/Normal Colon		184	76	106	34	72	42	33	56
Non-colorectal Cancer		36	22	14	4	5	14	8	65
Non-cancerous Disease		149	57	92	14	29	56	41	66
Total		547	266	279	57	141	180	140	63	53	16

¹Colorectal lesions are divided regarding their location into ‘Distal’: descending, sigmoid, left flexure, rectum and ‘Proximal’: cecum, ascending, right flexure, transverse.

²Non-colorectal Cancer include bladder, breast, liver, lung, prostate and pancreatic cancers.

³Non-cancerous Diseases include gastritis, hypertension, type II diabetes, liver disease, rheumatoid and non- rheumatoid arthritis, cholycystitis, cystitis, chronic heart disease, esophagitis, IBD, pancreatitis, chronic renal failure, respiratory infection.

⁴Total sample numbers can be higher than the sum of all subgroups when sample annotations are missing, for some patient samples SEPT9 methylation measurement was incomplete.

⁵Median patient age at time of sample collection in years.