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. 2008 Nov 21;3(11):e3787. doi: 10.1371/journal.pone.0003787

Figure 3. Recombinant SAP enzymatic activity determined by DNS acid assay (A) Determination of the activity of SAP recombinant forms on different a-glucan substrates.

Figure 3

The mixtures contained 1% (w/v) of each substrate dissolved in PBS. After incubation of recombinant SAP and a-glucans at 37°C for 1 h, DNS buffer was added and the release of reducing groups was determined by reading the absorption at 575 nm. The same sample without the enzyme was used to correct for non-enzymatic release of reducing sugars. (B) Comparison between recombinant SAP(H+L) and recombinant PulA for the capacity to degrade a-glucans. Experimental protocol as in (A). The data are the mean of 3 independent experiments ± SD. The asterisk indicates a significant difference between values (p<0,01).