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| Primer | Sequence (5′ to 3′) |
| Recombinant protein | |
| GBS5F | CTAGCTAGCGAAGAAGTAAGTGTTTCTC |
| GBS5R | CCCGCTCGAGATTAGCTTCATTTGTCAGA |
| COH1Δsap | |
| P1 | CCCGCTCGAGTCATCTACACACGCATTTTTCC |
| P2 | TCCAGTTTTTGGCAAGGGAGTATTTTGCAATGTAGATGG |
| P3 | TTGCAAAATACTCCCTTGCCAAAAACTGGAGATAAGTCA |
| P4 | CCCGCTCGAGTTCCTAATGCTGTCTTCCCAAC |
F corresponds to forward primer and R to reverse primer. Restriction sites are underlined.
