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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Virol Methods. 2008 Sep 17;153(2):253–256. doi: 10.1016/j.jviromet.2008.07.032

Fig. 1.

Fig. 1

QPCR primer sets and linear or circular HIV cDNA. HIV late reverse transcripts are measured by the primers MH531 and MH532. This amplicon spans the HIV primer binding site and is present in all forms of HIV cDNA, including linear cDNA, integrated provirus, 2-LTR circles, and 1-LTR circles. All complete reverse transcripts are measured by the primer set MH531 and MH532. 2-LTR circles are specifically amplified by the primers MH535 and KY268. The junction of U5 and U3 is unique to 2-LTR circles. The 2-LTR circle primer pair spans this unique junction in 2-LTR circles and will not amplify alternative HIV cDNA forms.