Standard curves from universal QPCR and fast QPCR. (A) Universal QPCR derived standard curves for HIV late reverse transcripts (left panel) and HIV 2-LTR circles (right panel). (B) Fast QPCR derived standard curves for HIV late reverse transcripts (left panel) and HIV 2-LTR circles (right panel). Triplicate samples of 102, 103, 104, 105, 106, 107, and 108 target molecules were amplified for 40 cycles. Best fit lines were generated by the SDS software. For all standard curves R2 > 0.995.