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. 2008 Nov 20;3(11):e3779. doi: 10.1371/journal.pone.0003779

Figure 4. RIFIN RNA expression during sexual development.

Figure 4

A: RT-PCR analysis of A- and B-type RIFINs in asexual and sexual stage parasites of the NF54 strain. RNA was harvested from 20–24 hour trophozoites (T) and at days 2, 4, 6, 8 and 10 of gametocytogenesis (D2–D10). RNA was reverse transcribed into cDNA (+) and PCR was performed with specific primers for A-RIFINs and B-RIFINs. RNA samples in which reverse transcriptase was omitted served as negative controls (−). Positive controls were performed on genomic DNA (g). B: RT-PCR analysis with specific primers detecting the A-type rif variant PFD1240w and the B-type rif variant PFI0025c. Stages are as in (A).