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. 2008 Nov 20;3(11):e3779. doi: 10.1371/journal.pone.0003779

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Petter et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: Distance tree of rif gene upstream regions (rups) from 3D7. The 5′-sequences containing 1500 bp upstream of each rif gene were aligned with ClustalW. Distance trees were generated using the p-distance/Neighbor-Joining method with pairwise deletion of gaps. The clusters rupsA1, rupsA2, rupsA/B and rupsB were verified by bootstrapping, and bootstrap values are indicated at the branches. The cluster rupsC could not be verified by bootstrapping. Star: RupsA2, rups A/B and rupsB variants with transcription orientation towards centromere, contrary to general transcription orientation. Hash: Upstream regions previously referred to as upsA-rif [48]. B: Chromosomal orientation of variants belonging to each of the rups clusters.