Table 2.

Summary of the inter-day accuracy and precision

	QC1		QC2		QC3		QC4		QC5
Nominal Conc	10 (ng/ml)	%RSD	25 (ng/ml)	%RSD	1.25 (µg/ml)	%RSD	12.5 (µg/ml)	%RSD	25 (µg/ml)	%RSD
G-CA	10.0	13.7	23.5	14.2	1.1	14.2	12.0	14.1	24.7	12.2
G-UDCA	9.1	10.6	25.1	9.8	1.3	9.8	13.0	10.3	27.3	5.2
G-CDCA	10.0	9.1	23.3	8.4	1.3	8.4	11.7	9.5	24.8	12.5
G-DCA	9.6	11.9	23.9	10.4	1.3	10.4	11.8	8.4	23.1	7.5
G-LCA	9.5	9.9	26.8	6.9	1.4	6.9	11.9	10.1	24.8	9.11
Nominal Conc	40 (ng/ml)	%RSD	100 (ng/ml)	%RSD	5 (µg/ml)	%RSD	50 (µg/ml)	%RSD	100 (µg/ml)	%RSD
T-MCA	42.8	7.1	102.4	13.2	5.1	13.2	47.8	9.8	98.8	9.2
T-CA	42.1	13.4	101.7	2.3	5.1	2.3	47.7	6.7	106.3	6.4
T-UDCA	44.7	3.27	116.5	2.85	5.5	2.85	55.9	2.45	103.9	16.03
T-CDCA	41.7	6.7	49.0	0.7	4.4	0.7	42.7	3.9	97.8	9.9
T-DCA	43.9	4.6	105.4	2.2	4.4	2.2	50.8	7.6	92.4	9.7
T-LCA	36.8	5.7	111.7	3.9	5.2	3.9	56.3	5	108.3	2.9
Nominal Conc	20 (ng/ml)	%RSD	50 (ng/ml)	%RSD	2.5 (µg/ml)	%RSD	25 (µg/ml)	%RSD	50 (µg/ml)	%RSD
αMCA	21.7	2.6	43.9	3.8	2.3	3.8	26.2	9	47.7	2.2
βMCA	17.1	14.9	47.5	3.8	2.5	3.8	21.4	6.2	45.0	1.7
CA	18.4	3.7	46.7	5	2.7	5	24.9	9.9	49.1	6.4
UDCA	19.9	2	49.2	5.7	2.5	5.7	26.4	3.3	51.7	4.3
CDCA	21.1	1.8	50.6	7.7	2.6	7.7	24.3	11.8	52.1	8.1
DCA	21.2	8.5	46.9	8.5	2.6	8.5	24.1	1.96	48.7	12
LCA	18.7	11.1	52.5	3.6	2.5	3.6	25.6	12.1	50.8	8.2

Results are calculated from the 3 validation trials of calibration curves prepared in bile.