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. Author manuscript; available in PMC: 2008 Nov 12.
Published in final edited form as: J Am Chem Soc. 2006 Apr 26;128(16):5324–5325. doi: 10.1021/ja060537y

Figure 1.

Figure 1

The principles of the QD-based nanosensor for Rev-RRE interaction assay. (a) Secondary structure of biotinylated RRE IIB RNA. Nucleotides identified as important for Rev binding are shown in red. (b) Sequence of Cy5-labeled Rev peptide. (c) Conceptual scheme of the QD-based nanosensor for Rev-RRE interaction assay based on FRET between 605QD and Cy5. The binding of a Cy5-labeled Rev to a biotinylated RRE IIB RNA formed a Rev-RRE complex, which was caught on the surface of a 605QD to form a 605QD/Rev-RRE/Cy5 assembly through specific streptavidin-biotin binding. FRET occurred between the 605QD and Cy5 upon illumination of the 605QD/Rev-RRE/Cy5 assemblies with an excitation wavelength of 488nm.