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. Author manuscript; available in PMC: 2008 Nov 12.
Published in final edited form as: J Am Chem Soc. 2006 Apr 26;128(16):5324–5325. doi: 10.1021/ja060537y

Figure 2.

Figure 2

Characterization of FRET in the 605QD/Rev-RRE/Cy5 assemblies. The 605QD fluorescence (■) and Cy5 fluorescence (□) were measured as a function of the Rev-RRE complex-to-605QD ratio. The direct excitation of equivalent amounts of Cy5-labeled Rev without the presence of 605QD (Δ) was shown for comparison. 605QD concentration: 38 nM; Rev-RRE complex concentration varied with the ratio of Rev-RRE complex to 605QD as shown. Error bars, S.D. (n=3).