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Pyk2 Binds to a Cam Agarose Affinity Column
αT3-1 whole-cell lysates were prepared (see Materials and Methods) and subjected to Cam agarose affinity chromatography in the presence or absence of Ca2+ followed by column elution with EGTA. Input lysates (20%; positive control for Western blots) and fractions 1–4 were resolved on SDS-PAGE, and Western blot analysis was used to determine the presence of IQGAP, Pyk2, and Src.
