Figure 4.

Modulation of NCoR Levels Differentially Influences Ligand-Dependent Transactivation of LXRα

A, NCoR knockdown. 293T cells were cotransfected with 5×UAS-SV40-luciferase and Gal-LXRα along with siRNA against human NCoR or nontargeting control (NTC). Cells were treated with ligands (10 μm) or vehicle overnight. Relative activation was determined as fold change vs. Gal-DBD controls. *, P < 0.05 or **, P < 0.01 compared with NTC. NCoR knockdown was confirmed by immunoblotting (inset), and Ran protein level is shown as loading control. B, NCoR overexpression. 293T cells were cotransfected with 5×UAS-SV40-luciferase and Gal-LXRα along with human NCoR expression vector or empty control plasmid. Cells were treated with ligands (10 μm) or vehicle overnight. Each bar is the mean value ± se (n = 3) from three independent experiments. NCoR expression was confirmed by immunoblotting (inset), and β-actin protein level is shown as a loading control. *, P < 0.05 compared with vector control for each ligand treatment calculated by Student’s t test. siNCoR, small interfering NCoR.