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Novel E2 Targets in Bone
A, U2OS-ERα cells were treated with 10 nm E2 for 3 h, and RNA was obtained. Quantitative PCR was performed in triplicate on cDNA from three independent experiments. B, Primary calvarial osteoblasts were treated with 10 nm E2 for 3 h, and RNA was obtained. Quantitative PCR was performed in triplicate on cDNA from three independent experiments. Error bars for all parts represent mean ± 1 sd. AP, Alkaline phosphatase.
