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A, Fasting plasma acylated ghrelin concentrations monitored overnight every 20 min. The indicated difference was determined by two-way ANOVA and Tukey’s test. B, Plasma acylated ghrelin concentrations monitored every 20 min beginning at 0600 h after initiation of ghrelin infusion of 0.15 μg/kg·h, which was doubled every 90 min to a maximal rate of 2.4 μg/kg·h. Data are the mean ± sem (n = 12, no-E2 group; n = 8, E2 group). MN, Midnight; NS, not significant.
