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. 2008 Oct 5;36(20):6372–6385. doi: 10.1093/nar/gkn620

Figure 6.

Figure 6.

Effect of siRNA against Stra13/DEC1. (A–C) Hepa1c1c7 cells were transfected with the siRNAs against Stra13/DEC1 as described. Before harvest, the transfected cells were exposed to hypoxia (1% O2, 24 h). The levels of mRNA in each sample were quantified by Q-PCR. Values represent means and standard deviations of three experiments. WB analysis was performed using anti-SREBP-1 antibody (Santa Cruz Biotechnology) and anti-HDAC1 antibody. (D) Hepa1c1c7 cells were incubated in hypoxic conditions (1% O2) for the indicated times. The levels of Stra13/DEC1 and DEC2 mRNA were analyzed by Q-PCR. The expression level of 18S rRNA was used for normalization. (E) 3T3-L1 cells were transfected with the siRNAs against Stra13/DEC1 as described. Before harvest, the transfected cells were exposed to hypoxia (1% O2, 24 h). The level of DEC2 mRNA in each sample was quantified by Q-PCR. Values represent means and standard deviations of three experiments.