Figure 5.

Thermal denaturations and circular dichroism spectra of the -Ψ₂₇ and the -Ψ₂₇;f⁵C₃₄. (A) Thermodynamic stability of the ASLs. UV-monitored, thermal data were averaged from three denaturations and two renaturations for the -Ψ₂₇;f⁵C₃₄ (thin gray line) and for the -Ψ₂₇ (thick black line). (B) Circular dichroism spectra. Spectra of the -Ψ₂₇;f⁵C₃₄ (thin gray line) and that of the -Ψ₂₇ (thick black line) at the approximately equal concentrations of 2 µM were collected over the wavelength range of 200 to 300 nm.