Figure 11.

Real-time PCR detection of the amplification of a 533-bp fragment of the DNA packaging protein of enterobacteria phage λ using TaqMan® probe detection. Reactions were performed in quadruplicate and compared the performance of unmodified and double OXP-modified primers for their ability to detect 10, 50, 100, 500, 1000, 5000 and 10 000 copies of λ genomic DNA. This figure displays the amplification plots for unmodified (A) and double OXP-modified (B) primers as well as the corresponding standard curve (C).