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. 2008 Sep 16;36(20):e131. doi: 10.1093/nar/gkn575

Figure 2.

Figure 2.

Conversion of OXP-modified primers to the corresponding PDE primer. (A) Reverse phase HPLC analysis of a single OXP-modified primer after 5 min incubation at 95°C in 50 mM KCl, 1.5 mM MgCl2 and 10 mM Tris (pH 8.4 at 25°C). (B) Reverse phase HPLC analysis of the double OXP-modified primer after 8 min incubation at 95°C in 50 mM KCl, 1.5 mM MgCl2 and 10 mM Tris (pH 8.4 at 25°C). Peaks which are due to primers containing a single OXP modification are labeled 1× OXP, while peaks with two OXP modifications are labeled 2× OXP.