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. 2008 Sep 16;36(20):e131. doi: 10.1093/nar/gkn575

Figure 3.

Figure 3.

Evaluation of the rate of unmodified primer formation after incubation of OXP-modified HIV-1 primers at elevated temperatures. (A) Kinetic curves of conversion of forward (filled circle) and reverse (filled square) single OXP-modified primers to corresponding PDE primers at 95°C in 1× PCR buffer (see, Figure 2). (B) A semi-logarithmic plot of kinetics of conversion of forward (open circle) and reverse (open square) single OXP-modified primers to a corresponding PDE primer at 95°C in 1× PCR buffer (see, Figure 2). (C) Kinetic curves of conversion of a double OXP-modified forward primer to the corresponding PDE primer in 1× PCR buffer at 95°C (see, Supplementary Scheme 3). Starting double OXP-modified primer (filled circle); intermediate isomeric single OXP-modified primers (filled square); and unmodified PDE primer (filled triangle) are indicated with the appropriate symbols.