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. 2008 Sep 16;36(20):e131. doi: 10.1093/nar/gkn575

Figure 5.

Figure 5.

Agarose gel analysis of PCR amplification of 365-bp fragment of HIV-1 DNA template using PDE, OXP and MAF primer sets at concentration of 0.5 μM, volume of 25 μl. Lanes 1–4 contain unmodified PDE primers, where lanes 1 and 2 are nontemplate control (NTC) and lanes 3 and 4 have five copies of HIV-1 recombinant DNA. Lanes 5–8 contain single OXP-modified primers, where lanes 5 and 6 are NTC and lanes 7 and 8 have five copies of HIV-1 recombinant DNA. Lanes 9–12 contain single MAF-modified primers, where lanes 9 and 10 are NTC and lanes 11 and 12 have five copies of HIV-1 recombinant DNA. The 50-bp ladder is loaded after lanes 4, 8 and 12. Thermal cycling parameters: 95°C (10 min), 40 cycles of [95°C (40 s), 56°C (30 s) and 72°C (2 min)].