Skip to main content
. 2008 Sep 16;36(20):e131. doi: 10.1093/nar/gkn575

Figure 8.

Figure 8.

Comparison of the performance of OXP-modified primers to other Hot Start DNA polymerases. (A) Agarose gel analysis of the PCR products resulting from the 35 thermal cycles of amplification of five copies of a 365-bp fragment from the HIV-1 tat gene using 0.5 μM unmodified, single OXP-modified and double OXP-modified primers. Reactions containing unmodified primers were amplified by Taq DNA polymerase, Platinum® Taq DNA Polymerase, AmpliTaq Gold® DNA Polymerase, HotStart-IT™ Taq DNA Polymerase and DyNazyme™ II Hot Start DNA Polymerase. Reactions containing single and double OXP-modified primers were amplified by Taq DNA polymerase. (B) Graphical representation of PCR amplicon yield. The results from triplicate experiments were averaged and are normalized to the yield of reactions containing single OXP-modified primers plus Taq DNA polymerase. Error bars represent the SD. (*), indicates Hot Start DNA polymerases.