Figure 9.

Real-time PCR analysis of the formation of a 365-bp amplicon of the HIV-1 tat gene using detection by SYBR Green® I nucleic acid stain. Reactions, which contained 10 ng of human genomic DNA and 0, 1, 5, 25 or 125 copies of HIV recombinant DNA as calibrated from the Gene Amplimer kit, were performed in quadruplicate and employed 0.5 μM unmodified, single OXP-modified and double OXP-modified primers. This figure displays amplification plots: (A) unmodified primers; (B) single OXP-modified primers; and (C) double OXP-modified primers; dissociation curves: (D) unmodified primers; (E) single OXP-modified primers; and (F) double OXP-modified and a standard curve (G).