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. 2008 Oct 21;36(20):6608–6619. doi: 10.1093/nar/gkn666

Figure 2.

Figure 2.

LMP1 enhances the expression of miR-155 in an EBV-negative B-cell line. (A) A representative northern blot analysis on total RNAs obtained from DeFew cells transfected (TF) as indicated or untransfected (UT). The blot was analyzed for the expression of mature miR-155 and normalized by the ubiquitous small nuclear RNA (U6). (B) Graphic representation of the northern blot results as average of three independent experiments. (C) Western blot analysis of LMP1 expression in the transfected cells compared to MC3 cell immortalized by EBV. Input proteins were equalized by detecting the endogeneous γ-tubulin. (D) Northern blot analysis of total RNAs obtained from DeFew cells at 72 h post-infection with retroviral vector expressing LMP1 or empty vector. The blot was analyzed for the expression of mature miR-155 and normalized by U6. (E) Graphic representation of the northern blot results as average of three independent experiments. (F) Western blot analysis of the expression of LMP1 upon infection with the retroviral vector. Input proteins were equalized by detecting the endogeneous γ-tubulin.