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. 2008 Oct 21;36(20):6608–6619. doi: 10.1093/nar/gkn666

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Semi-quantitative ChIP on the BIC promoter at indicated times in DeFew cells after retroviral infection with a vector expressing LMP1 or empty vector. ChIP assay of chromatin extracted at the indicated times from DeFew cells infected with pBABEpuroLMP1 or empty vector. (A) left panel: After immunoprecipitation with the indicated antibodies, the DNA samples were purified and subjected to PCR with specific primers amplifying the NF-κB site 1 region or NF-κB site 2 region. MW = molecular weight marker; p65 = pAB anti NF-κB p65; ti NF; H3 = pAb anti Histone H3. Right panel: After immunoprecipitation, the DNA samples were purified and subjected to PCR with specific primers amplifying the G3PDH promoter region. (B) Graphic of the results showed in panel (A; C) After immunoprecipitation with mAb directed against the RNA pol II, the DNA samples were purified and subjected to PCR with specific primers amplifying the TATA box region. MW = molecular weight marker; pol II = mAB anti RNA pol II; H3 = pAb anti Histone H3.