Figure 9.
PU.1 protein levels correlate with LMP1-mediated activation of miR-155 expression (A) western blot analysis of PU.1 expression in EBV-immortalized B cells (MC3) EBV-negative B cells (DeFew) infected with pBABEpuroLMP1 (DeFew LMP1) or pBABEpuro, and selected with 1 mg/ml of puromycin for 7 days (DeFew Empty Vector); (B) Graph of densitometric analysis of A normalized by γ-tubuline; (C) northern blot analysis of mir-155 expression in EBV-immortalized B cells (MC3) EBV-negative B cells (DeFew) infected with pBABEpuroLMP1 (DeFew LMP1) or pBABEpuro (DeFew Empty Vector) and selected with 1 mg/ml of puromycin for 7 days; (D) Graph of densitometric analysis of C normalized by U6.
