Table 3.
Bases incorporated opposite O6-meG in the truncated RNA during transcription with T7 RNAP and hRNAPIIa
Sequence | RNA length (bases) | Percentage | |
---|---|---|---|
Expected RNA sequence | …UCUUGCGGUAGCGAUGG | ||
Sequence obtained with T7 RNAP | …UCUUGCGGUAG | 272 | 25 |
…UCUUGCGGUAGA | 273 | 25 | |
…UCUUGCGGUAGCAA | 275 | 50 | |
Sequence obtained with hRNAPII | …UCUUGCGGUAG | 272 | 80 |
…UCUUGCGGUAC | 20 |
aBold, underscored bases represent misincorporated bases after the lesion.