Figure 2.

RS1 promoter induction levels by CRX, OTX2, NR2E3 and NRL. HEK293 cells were cotransfected with the −177/+32 RS1 promoter–reporter construct and transcription factor expression vectors. X-fold stimulation values were calculated by normalizing CRX, OTX2, NRL and NR2E3 cotransfected cells to mock-vector transfections. CRX (black bars) specifically induces the RS1 promoter in a dose-dependent manner. OTX2 (dark gray bars), NR2E3 (light gray bars) and NRL (white bars) do not induce or suppress the RS1 promoter. Each transfection was repeated three times with duplicate wells analyzed. Error bars represent the standard deviation of the mean from protein normalized luciferase activities.