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. 2008 Oct 4;36(20):6363–6371. doi: 10.1093/nar/gkn678

Figure 2.

Figure 2

(A) Time course of RNase H hydrolysis profile with the unmodified RNA/DNA hybrid. Time points are: 0 s (lane a); 30 s (lane b); 1 min (lane c); 2 min (lane d); 3 min (lane e); 4 min, (lane f); 5 min (lane g); 6 min (lane h); 7 min (lane i); 8 min (lane j); 9 min (lane k) and 10 min (lane l). (B) RNase H cleavage for RNA/DNA hybrids containing single methylphosphonate substitution in the DNA primer. Lane a represents wild-type substrate without enzyme, and lane b wild-type substrate with enzyme. lane c, −10; lane d, −11; lane e, −12; lane f, −13; lane g, −14; lane h, −15; lane i, −16; lane j, −17; lane k, −18; lane l, −19 and lane m, −20. The time point for the hydrolysis of these substrates is 10 min.