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Oct4 binding by the OPE in trapped ESCs. Chromatin immunoprecipitation was performed as described in the Methods section using anti-Oct4 and anti-V5 antibodies. PCRs of the precipitated and purified DNAs were performed with osteopontin gene- and gene-trap-specific primers. P048G09, parental clone with enhancer; P048G09 G1, recombined subclone without enhancer; MW, molecular weight standard (1 kb plus ladder, Invitrogen).
