Fig. 4.
Antigen-specific central memory T-cell responses in splenocytes induced by topical DermaVir prime/VacV boost regimen. Mice (four per group) were topically immunized at weeks 0 and 2 with control pVax or pGag DermaVir, either alone or co-formulated with pIL-7 or pIL-15 (25 µg total DNA/immunization) and boosted at week 6 with 2.2 × 106 PFU VacV-Gag (i.p.). IFN-γ production in response to four HIV-1 Gag peptide pools was evaluated at week 8 in total splenocytes by cultured ELISPOT. Net number of spots was determined by subtracting the number of spots formed in control medium wells.