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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Immunol. 2008 Oct 1;181(7):4908–4917. doi: 10.4049/jimmunol.181.7.4908

FIGURE 2.

FIGURE 2

IFN-γ synergizes with sVSG to induce transcription of IRF-7 and IFN-β in macrophages. RAW 264.7 macrophages were pretreated with IFN-γ (γ, 20 U/ml) or medium alone (M) for 24 h followed by treatment with CpG-ODN (O, 10 μg/ml), IFN-γ alone, or different concentrations of sVSG for 6 h as shown. Total RNA was harvested from cell monolayers by phenol-chloroform extraction and each sample was analyzed by RT-PCR for changes in IRF-7 and IFN-β expression. Changes in gene expression were assessed by resolving 10 μl of sample on 1% agarose gels containing ethidium bromide. The housekeeping gene G3PDH served as a loading control, and densitometric analysis was used to verify differences. Results shown are from one of four representative and independent experiments.