Figure 1. Increased cytokine production in response to L. pneumophila containing a functional T4SS compared to dotA mutants.

(A) ELISA measurements of cytokine levels in the BALFs of WT mice 24 hours following intranasal infection with PBS vehicle control or 5×10⁶ CFUs of WT, ΔflaA, or ΔdotA L. pneumophila on the thyA background. Each point represents an individual mouse. Lines indicate the mean cytokine levels for each group of mice. (B) ELISA measurement of cytokine production in WT bone marrow-derived macrophages infected with WT, ΔdotA, or ΔflaA L. pneumophila on the thyA background at an MOI = 5 for 24 hours. Data represent the mean±standard error of the mean (SEM) of the assay performed in triplicate and are representative of at least three independent experiments. P-values derived from two-tailed student's T test. * represents p<0.05. ** represents p<0.01. (C) Immunoblot analysis of pro-IL-1β production in WT bone marrow-derived macrophages infected with WT, ΔdotA, or ΔflaA L. pneumophila on the thyA background at an MOI = 5 for 24 hours. Blots were reprobed for analysis of total actin (loading control). Data are representative of at least two independent experiments.