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. 2008 Nov 28;4(11):e1000220. doi: 10.1371/journal.ppat.1000220

Figure 4. L. pneumophila type IV secretion induces a MyD88- and RIP2-independent transcriptional response.

Figure 4

(A) Venn diagram showing genes transcriptionally induced two-fold or more in response to the T4SS in Myd88−/−Trif−/− or Myd88−/−Rip2−/− macrophages infected for four hours with WT L. pneumophila compared to those infected with the ΔdotA mutant on the thyA background at an MOI = 25 versus genes transcriptionally induced two-fold or more at four hours following ISD transfection of Myd88−/−Trif−/− macrophages [25]. (B) Graph representing the percentage of genes transcriptionally induced two-fold or more upon infection with WT L. pneumophila compared to the ΔdotA mutant on the thyA background at an MOI = 25 for four hours in both Myd88 −/− Trif −/− and Myd88 −/− Rip2 −/− macrophages and that belong to various functional classes. (C) Quantitative RT-PCR analysis of Myd88 −/− Rip2 −/− macrophages infected with WT or ΔdotA mutant L. pneumophila at an MOI = 25 for four hours. Data are representative of at least two independent experiments.