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Detection of SP cells in adult mouse thyroid. Hoechst 33342 staining of mouse thyroid cell suspensions revealed the SP pattern of staining within the gated area (A), which was eliminated by treatment with verapamil (B). For some experiments, the SP fraction was further subdivided into SP1 and SP2 by setting a gate where the number of cells in the two regions becomes equal (C). Note that AB vs. C are two different thyroid preparations.
