Fig. 4.

ABCG2 expression in adult mouse thyroid by in situ hybridization and immunofluorescence. A, In situ hybridization with sense (SS) and anti-sense Abcg2 probes (AS) was performed to locate the expression of Abcg2. Positive signals were detected only in cells surrounding thyroid follicles, but not cells lining follicles (lower panel). B, Immunofluorescence revealed that some cells are positive for only ABCG2 or Sca1, whereas others are double positive for both genes. Only representative areas are shown in which double-positive cells are present. A, upper panel: magnification, ×200; bar, 100 μm. A, lower panel, B: magnification, ×400; and bar, 50 μm.