TABLE 1.
Primer sequences used for real-time PCR
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | TaqMan probe (5′–3′) |
|---|---|---|---|
| Abcg2 | CTGGTCCTCTCCCTGCTTTTTC | GCCGTTGTTGTTTCTCTGCG | |
| Nucleostemin | TCGGAGTCCAGCAAGCATTG | GCAGCACTTTCCACATTTGGG | |
| Oct4 | ATCGGACCAGGCTCAGAGGTATTG | TTCTCCAACTTCACGGCATTG | |
| Titf1 | GCGCCGGGTGCTCTTC | CGTGGGTGTCAGGTGAATCA | (6FAM)CCGGAGCGCGAGCATCTGG (TAMRA) |
| Pax8 | AAAGGCCCCTCCTAGTTGATTC | CCGGCGATGCCTCACA | |
| Tpo | GGAAGTGACAAGGCACCTCATTC | GGGGAAGCAAGGATTTTGGTTC | |
| Thyroglobulin | TCAGGAAGGCACTGCTTATGG | GCCCTCTCTGGGCTGATAATT | |
| TSHr | AAGACACCTGGACTCTCAAAAAGC | AGAGAGGTCAGCCCGAGTGA | (6FAM)CCCGCTGTCGTTGAGTTTCCTCCAC (TAMRA) |