Figure 5.
Interferon (IFN) beta blockade partially diminishes MDA7/IL-24-induced cell death. MeWo melanoma cells were exposed to a dose titration of affinity-purified MDA7/IL-24 in the presence of neutralizing antibodies to IFN beta and assessed for cell death after 5 days. Gray bars = percentage of living cells, and black bars = percentage of dead cells (trypan blue positive) based on total cells. Anti-IFN beta–neutralizing antibody was used at 5× molar excess for predicting the level of IFN beta in supernatant with the IgG control added at the same concentration. Error bars represent one standard deviation of the mean.