Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Nov 17;183(4):681–696. doi: 10.1083/jcb.200803129

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Gonzalvez et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Caspase-8 activity is abrogated in tafazzin-deficient cells. (A) Control lymphoblastoid cells (DB037, lanes 1 and 3; DB015, lanes 2 and 4) and Barth syndrome–derived lymphoblastoid cells (DB105.2, lanes 5 and 7; DB105.3, lanes 6 and 8) were either untreated or treated with anti-Fas antibody (Fas) for 14 h. Total cells extract was analyzed by Western blotting for Bid levels. After digital scanning of the immunoblot, the intensities of Bid levels as a percentage of actin levels were plotted (bottom). (B) Control (shCont1) and tafazzin-knockdown (shTaz1) HeLa cells were either untreated or treated with anti-Fas antibody for 14 h, and analyzed as in A. (C) Mitochondria were isolated from the indicated cells treated as in B, and the tBid levels on the mitochondria were analyzed by Western blotting. Subunit 6 of complex I was used as a mitochondrial marker and loading control. After digital scanning of the immunoblot, the intensities of tBid levels as a percentage of complex I levels were plotted (bottom). (D and E) The indicated control or tafazzin-deficient cells were treated as in A and analyzed for IETDase (caspase-8–like) activity. Error bars represent ± standard deviation.