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. 2008 Nov 17;183(4):667–680. doi: 10.1083/jcb.200805163

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© 2008 Huang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Phospho-BubR1 antibodies delay anaphase onset in vivo. (A) Select frames from time-lapse videos of HeLa H2B-GFP that were injected with the indicated antibodies. (B) Videos were analyzed frame by frame to determine the percentage of cells from NEB to anaphase onset. For pS670, n = 20; pS1043, n = 20; T1042, n = 30; BubR1, n = 12; control IgG, n = 23. Error bars represent the highest and lowest values for each timepoint. (C) Cells injected with nonimmune and pS670 IgG were cold treated and stained for ACA and tubulin to visualize kinetochore attachments (insets).