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. 2008 Nov 17;183(4):667–680. doi: 10.1083/jcb.200805163

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© 2008 Huang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 7. — Mps1 is required to phosphorylate BubR1 in cells. Cells transfected with control, and Mps1 siRNA were released from a double thymidine block. MG132 was added 8 h later, and mitotic cells were harvested 2 h later by shakeoff. Lysates were probed with all four phospho-BubR1 antibodies. Two different amounts of control lysates were used, so it was possible to directly compare the band intensities with Mps1-depleted lysate. CENP-F, BubR1, and cyclin B1 were used as loading controls.