Figure 1.

The USF proteins bind the endogenous Fshr promoter in expressing cells. Interactions between the USF proteins and Fshr promoter, in vivo, were evaluated using ChIP with cross-linked chromatin from primary Sertoli cells and granulosa cells isolated from wild-type immature (21 d old) mice (A) or 15-d-old primary rat Sertoli and peritubular myoid cells (B). In DNA isolated from each immunoprecipitated chromatin or input sample, the promoter region (upper) and a negative control region (lower) located 5000 bp (mouse) or 6000 bp (rat) upstream of exon 1 were amplified by PCR. Immunoprecipitations for USF1 or USF2 were compared with immunoprecipitations with a control antibody against MYC, which does not bind the Fshr E-box, in vitro, and with normal rabbit IgG. Chromatin samples and antibodies used for immunoprecipitation are noted above each ethidium bromide-stained agarose gel.