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Estrogen is required for daily changes in activational state of RFRP-ir cells. Shown is the mean (±sem) percentage of RFRP-ir cells expressing FOS in ovariectomized hamsters implanted with estradiol or a blank capsule during the LH surge (2000 h) or after the LH surge (2300 h). *, Significantly different from all other values, P < 0.05.
