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. 2008 Jul 19;149(10):5235–5243. doi: 10.1210/en.2008-0610

Figure 1.

Figure 1

Effects of PPI2, endothall, and cyclosporine A (CsA) on cell survival. HT-22 cells and C6-glioma were seeded into 96-well plates at a density of 3500 cells/well. Primary cortical neurons were seeded into 96-well plates at a density of 25,000 cells/well. A, HT-22 cells were treated with varying concentrations of PPI2, endothall, and/or cyclosporine A. B, C-6 cells were treated with varying concentrations of PPI2, endothall, and/or cyclosporine A. C, Primary cortical neurons were treated with varying concentrations of PPI2, endothall, and/or cyclosporine A. Cell viability was determined by calcein AM assay (Molecular Probes) after 24 h exposure to the various compounds. All data were normalized to percent survival of nontreated control. Depicted are mean ± sem for 10 independent experiments with two replicates per experiment. DMSO, Dimethyl sulfoxide. *, P < 0.05 vs. vehicle control; †, P < 0.05 vs. two combination treatment.