Figure 2.

Effects of PPI2, endothall, or cyclosporine A on 17β-estradiol-mediated neuroprotection in primary cortical neurons. Primary cortical neurons were seeded into 96-well plates at a density of 25,000 cells/well. A, Cells were treated simultaneously with 200 nm PPI2, 50 μm glutamate, and/or 100 nm 17β-estradiol. B, Cells were treated simultaneously with 9 μm endothall, 50 μm glutamate, and/or 100 nm 17β-estradiol. C, Cells were treated simultaneously with 500 nm CsA, 50 μm glutamate, and/or 100 nm 17β-estradiol. Cell viability was determined by calcein AM assay (Molecular Probes) after 24 h exposure to the various compounds. All data were normalized to percent survival of nontreated control. Depicted are mean ± sem for 10 independent experiments with two replicates per experiment. *, P < 0.05 vs. vehicle control; †, P < 0.05 vs. glutamate-treated group.