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. 2008 Nov 21;3(11):e3788. doi: 10.1371/journal.pone.0003788

Figure 1. Analysis of feeder cell contamination during neuronal differentiation of murine embryonic stem cells.

Figure 1

Feeder cells were transfected with the neomycine resistance (nr) gene. A. RT-CR and nested RT-PCR analysis of nr gene expression (NR) during neuronal differentiation of murine ESCs. Displayed are images of representative results following RT-PCR analysis. GAPDH was used as a control. B. Percentage of feeder cell contamination was evaluated by nr gene expression analysis of predefined ratios of stem and feeder cells. Displayed are representative results of PCR products. C. Following logarithmic transformation signal density is plotted as a linear function against feeder cell number (n = 4).