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. 2008 Nov 21;3(11):e3788. doi: 10.1371/journal.pone.0003788

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Schneider et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Schematic representation of embryoid body formation: a. trypsination of stem/feeder cells, b. standard protocol for embryoid body formation in hanging drops, c. automated harvesting of feeder-freed stem cells colony fractions, d. transfer of feeder-freed stem cell to V-96 plate. B. Microscopic images of time dependent growth of (feeder-freed) f-f EBs and (standard) stEBs.