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. 2008 Nov 28;4(11):e1000280. doi: 10.1371/journal.pgen.1000280

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Johnson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Diagram of epitope-tagged SUVH9 and SUVH2. The TAG is either 3xHA or 9xMyc as described in Material and Methods. B. Quantitative RT-PCR of SDC gene relative to ACTIN in suvh9 suvh2 kyp untransformed and transformed with myc-tagged SUVH9. C. Complementation of suvh2 was tested by MEA-ISR southern blot using MspI digested DNA isolated from Columbia (wt), drm1drm2, suvh2, suvh9, and suvh2 transformed with HA-tagged SUVH2. D. Western blot of HA- tagged SUVH2 and SUVH9 under control of their endogenous promoters in transgenic plants. Input refers to leaf extract before immunoprecipitation, IP after immunoprecipitation. E. Expression levels of SUVH9 and SUVH2 from different tissues as measured on microarrays.